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D2532

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Synonym(s):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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Pack SizeSKUAvailabilityPrice
5 mL
Please contact Customer Service for Availability
PLN 678.00
5 x 5 mL
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PLN 2,530.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

PLN 678.00

Please contact Customer Service for Availability
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Quality Level

300

form

solution

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Preparation Note

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

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This Item
D9905S4030H7033
Denhardt’s Solution 50x for Northern and Southern blotting, solution

Sigma-Aldrich

D2532

Denhardt′s Solution 50x

Denhardt’s Solution for Northern and Southern blotting, lyophilized powder

Sigma-Aldrich

D9905

Denhardt′s Solution

Dextran Sulfate 50% Solution

Millipore

S4030

Dextran Sulfate 50% Solution

PerfectHyb™ Plus Hybridization Buffer for Northern and Southern blotting, solution

Sigma-Aldrich

H7033

PerfectHyb™ Plus Hybridization Buffer

technique(s)

Southern blotting: suitable

technique(s)

Southern blotting: suitable

technique(s)

hybridization: suitable (nucleic acid)

technique(s)

-

Quality Level

300

Quality Level

100

Quality Level

100

Quality Level

200

form

solution

form

lyophilized powder

form

-

form

solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

storage temp.

room temp

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

-

foreign activity

-

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000484704
0000484704
0000411631
0000411631
0000375543

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Protocols

Protokoły Northern i Southern Blot

Poznaj podstawy blottingu Northern i Southern, wraz z protokołami i aplikacjami do przenoszenia makrocząsteczek na nośniki membranowe.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

View All Protocols

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Global Trade Item Number

SKUGTIN
D2532-5X5ML04061838255143
D2532-5ML04061838104885

Questions

  1. Can you share the CAS # for the components (Ficoll and PVP) that are used to make the 50X Denhart's Solution?

    1 answer

    1. The CAS number for the Ficoll ingredient is 26873-85-8. The CAS number for the PVP ingredient is 9003-39-8 2.

      Below is the link to the Product Data Sheet for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/172/670/d2532dat.pdf

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