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73144

Resorufin

95%

Synonym(s):

7-Hydroxy-3H-phenoxazin-3-one, NSC 12097

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Size/SKUAvailabilityPrice
20 mg
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PLN 852.00

About This Item

Empirical Formula (Hill Notation):
C12H7NO3
CAS Number:
Molecular Weight:
213.19
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
211-241-8
Beilstein/REAXYS Number:
174850
MDL number:

PLN 852.00


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Quality Segment

assay

≥95.0% (HPLC), 95%

form

solid

mp

>300 °C (lit.)

storage temp.

−20°C

SMILES string

O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2

InChI

1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H

InChI key

HSSLDCABUXLXKM-UHFFFAOYSA-N

General description

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.
Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H2O2 by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

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This Item
R4883R3257R4758
form

solid

form

powder

form

powder

form

powder

assay

≥95.0% (HPLC), 95%

assay

~95%

assay

-

assay

≥90%

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

mp

>300 °C (lit.)

mp

-

mp

>300 °C (lit.)

mp

-


pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. what is solubility of resorufin

    1 answer
    1. The solubility of this product has not been determined. However, various sources state that this material is soluble in DMSO and only sparingly soluble in water. The exact concentrations are not reported.

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