46940

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

suitable for fluorescence, ≥75% (HPLC)

• Synonym(s): Fluorescein-5(6)-carboxamidocaproic acid N-succinimidyl ester
• Empirical Formula (Hill Notation): C₃₁H₂₆N₂O₁₀
• CAS Number: 114616-31-8
• Molecular Weight: 586.55
• NACRES: NA.32
• PubChem Substance ID: 329757270
• UNSPSC Code: 12352108
• MDL number: MFCD00467542

Properties

• Quality Segment: 100
• assay: ≥75% (HPLC)
• form: solid
• solubility: DMF: soluble, methanol: soluble
• fluorescence: λ_ex 490 nm; λ_em 514 nm (pH 8.2), λ_ex 491 nm; λ_em 515 nm in 0.1 M Tris pH 9.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)C(=O)NCCCCCC(=O)ON6C(=O)CCC6=O)c1.Oc7ccc8c(Oc9cc(O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)NCCCCCC(=O)ON%12C(=O)CCC%12=O)c7
• InChI: 1S/2C31H26N2O10/c34-18-6-9-22-24(15-18)41-25-16-19(35)7-10-23(25)31(22)21-8-5-17(14-20(21)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37;34-18-6-9-21-24(15-18)41-25-16-19(35)7-10-22(25)31(21)23-14-17(5-8-20(23)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h2*5-10,14-16,34-35H,1-4,11-13H2,(H,32,39)
• InChI key: WAGYELYAQOYZFU-UHFFFAOYSA-N

Description

General description

6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester (FNHS) is a fluorescent label, also known as fluorescein. FNHS max. excitation is at 490 nm with emission max. at 514nm.[1] 6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester binds covalently with primary amines.[2]

Application

6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester (FHS) is used as a reagent to conjugate the fluorophore 6-[Fluorescein-5(6)-carboxamido] hexanoic acid to molecules.6-[Fluorescein-5(6)-carboxamido] hexanoic acid is used as a fluorescein-based fluorescent label for proteins that can be analyzed in applications such as quenching experiments.

Reagent for introducing the fluorescein label; the spacer renders the reactive group more accessible to nucleophiles on biopolymers

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315 - H319 - H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves