40809

Purine nucleoside phosphorylase

recombinant, expressed in E. coli, ≥120 U/mL

• CAS Number: 9030-21-1
• UNSPSC Code: 12352204
• EC Number: 2.4.2.1 (BRENDA, IUBMB)
• MDL number: MFCD00131739

Properties

• recombinant: expressed in E. coli
• form: buffered aqueous solution (100 mM phosphate buffer with 1 mM MgCl₂)
• specific activity: ≥120 U/mL
• storage temp.: −20°C
• InChI: 1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1
• InChI key: MRWXACSTFXYYMV-FDDDBJFASA-N

Description

Application

Purine nucleoside phosphorylase is used to study nucleotide salvage pathways and purine metabolism. It is used to study purine nucleoside phosphorylase deficiency and responsible PNP genetic mutations.

Biochem/physiol Actions

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine. Mutations in the PNP gene are responsible for purine nucleoside phosphorylase deficiency.

Other Notes

1 U will cause the phosphorolysis 1 μmol of inosine to hypoxanthine and ribose 1-phosphate per minute at pH 7.4 at 25°C

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable