32855

Eosin Methylene blue

according to May-Grünwald

• UNSPSC Code: 12171500
• NACRES: MA.02
• PubChem Substance ID: 329754361
• MDL number: MFCD00005038
• Colour Index Number: 45380/52015

Properties

• form: powder or crystals
• quality: according to May-Grünwald
• εmax: ≥1000 at 635-655 nm in methanol, ≥650 at 512-532 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
• InChI: 1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1
• InChI key: NXBZXYJXNIFCRJ-UHFFFAOYSA-M

Description

General description

Eosin has been used in combination with methylene blue, to detect carboxlic acid group in organic compounds. It gives a red or orange-coloured precipitate on treatment with carboxylic acid solution in water, acetone, alcohol, or dioxin. It is also used as an indicator for differentiation of the colon from the typhoid-dysentery group of bacteria.

Mixture of eosin and methylene blue stains for use in differential microbiological media to culture and identify pathogenic microorganisms, e.g., Neisseria sicca,, Acinetobacter calcoaceticus.

Application

Eosin methylene blue was used for staining cells, during optical microscopy studies, done for analysis of cell morphology.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317,H319
• pcodes: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves