03899

Prussian blue soluble

• Empirical Formula (Hill Notation): C₆Fe₂KN₆ · xH₂O
• CAS Number: 12240-15-2
• Molecular Weight: 306.89 (anhydrous basis)
• UNSPSC Code: 12171500
• PubChem Substance ID: 329747831
• NACRES: NA.47
• MDL number: MFCD00135664

Properties

• form: powder
• solubility: water: 0.1 g/10 mL, blue to very deep blue
• εmax: ≥120 at 253-263 nm in water
• suitability: suitable for microscopy
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [K+].[Fe++].N#C[Fe-3](C#N)(C#N)(C#N)(C#N)C#N
• InChI: 1S/6CN.2Fe.K/c6*1-2;;;/q;;;;;;-3;+2;+1
• InChI key: ZJSIHHBKYVAMOE-UHFFFAOYSA-N

Description

Application

Prussian blue is not a dye but an inorganic pigment containing ferric ferrocyanide. Procedures have been published for the demonstration of non-hemoglobin, cytoplasmic iron, according to Sundberg and Broman,[1] and for the demonstration of hemosiderin according to Highman and from Lille and Fullmer based the work originally done by Perle in 1867. Prussian Blue is formed in situ in both of these methods. In combination with iron particles such as superparamagnetic iron oxide nanoparticles, Prussian blue may be used for tracking of transplanted cells such as mesenchymal stem cells.[2][3] Prussian blue may also be used to differentiate slow versus fast cycling cells by methods that monitor iron oxide particle labeling dilution thru cell division.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)