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Merck

01673

Acriflavine

BioReagent, suitable for fluorescence, ≥90% (AT)

Synonym(s):

3,6-Diamino-10-methylacridinium chloride mixt. with 3,6-diaminoacridine (proflavine), Euflavine, Trypaflavine Neutral

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10 G

PLN 383.00

50 G

PLN 1,260.00

PLN 383.00

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About This Item

Empirical Formula (Hill Notation):
C14H14ClN3
CAS Number:
8048-52-0
Molecular Weight:
259.73
UNSPSC Code:
12171500
PubChem Substance ID:
329747342
NACRES:
NA.32
MDL number:
MFCD00064307

Key Documents

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product line

BioReagent

Quality Level

100

assay

≥90% (AT)

form

powder

composition

Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC

solubility

H2O: 0.33 g/mL (lit.)(lit.)

fluorescence

λex 463 nm; λem 490 nm in ethanol

suitability

suitable for fluorescence

SMILES string

[Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46

InChI

1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H

InChI key

PEJLNXHANOHNSU-UHFFFAOYSA-N

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1 of 4

This Item
A8126A8251SML3350
Acriflavine BioReagent, suitable for fluorescence, ≥90% (AT)

Sigma-Aldrich

01673

Acriflavine

Acriflavine fluorescent label

Sigma-Aldrich

A8126

Acriflavine

Acriflavine hydrochloride fluorescent dye

Sigma-Aldrich

A8251

Acriflavine hydrochloride

Acriflavine purified ≥95% (HPLC)

Sigma-Aldrich

SML3350

Acriflavine purified

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

fluorescence

λex 463 nm; λem 490 nm in ethanol

fluorescence

-

fluorescence

-

fluorescence

-

solubility

H2O: 0.33 g/mL (lit.)(lit.)

solubility

H2O: 0.33 g/mL (lit.)(lit.)

solubility

H2O: 1 mg/mL

solubility

DMSO: 2 mg/mL, clear

form

powder

form

powder

form

powder

form

powder

assay

≥90% (AT)

assay

-

assay

-

assay

≥95% (HPLC)

composition

Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC

composition

-

composition

Acriflavine HCL content, 10-50% , Proflavine HCL, 25-70%

composition

-

Application

Acriflavine is used to fluorescently label high molecular weight RNA molecules .
Excitation and emission wavelengths in various solvents :
  • Methanol: λex = 424 nm; λem = 518 nm
  • Ethanol: λex = 426 nm; λem = 524 nm
  • Propanol: λex = 430 nm; λem = 512 nm
  • Butanol: λex = 430 nm; λem = 526 nm
  • Formamide: λex = 434 nm; λem = 524 nm
  • Glycerol: λex = 432 nm; λem = 540 nm
  • Water: λex = 416 nm; λem = 514 nm

Insoluble in ether, chloroform, and fixed oils. Utilized in fluorescence steady state measurements as a donor molecule (when paired with rhodamine 6G as the acceptor) to function as a pH sensor .

Other Notes

DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously[1]

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN6869
BCCN3404
BCCJ6459
BCCF5485
BCCB1935

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M Stöhr et al.
Stain technology, 53(4), 205-215 (1978-07-01)
Eight fluorescent dye combinations for simultaneous DNA-protein staining have been evaluated spectroscopically and flow microfluorometrically: propidium iodide (PI) with fluoresceinisothiocyanate (FITC), fluorescamine (FC), and dansylchloride (DANS); diamidinophenylindole (DAPII) with sulphorhodamin (SR101), tetramethylrhodamin isothiocyanate (TRITC), and nitrobenzodiazole (NBD); acriflavine (AF) with
Zheqing Cai et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(43), 17462-17467 (2013-10-09)
Both preclinical and clinical studies suggest that brief cycles of ischemia and reperfusion in the arm or leg may protect the heart against injury following prolonged coronary artery occlusion and reperfusion, a phenomenon known as remote ischemic preconditioning. Recent studies
Jessica E S Shay et al.
Carcinogenesis, 35(5), 1067-1077 (2014-01-11)
Hypoxia-inducible factors (HIFs) accumulate in both neoplastic and inflammatory cells within the tumor microenvironment and impact the progression of a variety of diseases, including colorectal cancer. Pharmacological HIF inhibition represents a novel therapeutic strategy for cancer treatment. We show here
Yael Gros et al.
The Journal of biological chemistry, 285(7), 5076-5084 (2009-12-17)
The vesicular neurotransmitter transporter VMAT2 is responsible for the transport of monoamines into synaptic and storage vesicles. VMAT2 is the target of many psychoactive drugs and is essential for proper neurotransmission and survival. Here we describe a new expression system
Shizuka Nakayama et al.
Chemical communications (Cambridge, England), 47(16), 4766-4768 (2011-03-15)
The ubiquitous bacterial biofilm regulator, c-di-GMP can form G-quadruplexes at physiological conditions in the presence of some aromatic compounds, such as acriflavine and proflavine. The fluorescence of these compounds is quenched upon c-di-GMP binding and some of the formed c-di-GMP
View All Related Papers

Global Trade Item Number

SKUGTIN
PH000036-25MG04061831294828
01673-10G04061838609571
01673-50G04061838609588

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