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B6506

Benzamidine hydrochloride hydrate

≥99%

Synonym(s):

Amidinobenzene hydrochloride, Benzamidinium chloride, Benzenecarboximidamide hydrochloride

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Size/SKUAvailabilityPrice
5 g
Please contact Customer Service for Availability
PLN 159.00
25 g
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PLN 478.00
100 g
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PLN 1,240.00

About This Item

Linear Formula:
C6H5C(=NH)NH2 · HCl · xH2O
CAS Number:
Molecular Weight:
156.61 (anhydrous basis)
UNSPSC Code:
12352107
NACRES:
NA.77
PubChem Substance ID:
EC Number:
216-795-4
Beilstein/REAXYS Number:
3594959
MDL number:

PLN 159.00


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biological source

synthetic (organic)

Quality Segment

assay

≥99%

form

powder

mp

86-88 °C (lit.)

solubility

water: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

Cl[H].[H]O[H].NC(=N)c1ccccc1

InChI

1S/C7H8N2.ClH.H2O/c8-7(9)6-4-2-1-3-5-6;;/h1-5H,(H3,8,9);1H;1H2

InChI key

BQVJCMMLDJPBFZ-UHFFFAOYSA-N

Application

Benzamidine hydrochloride hydrate has been used:
  • in western blot analysis
  • in immunoprecipitation
  • in the purification of yeast dynein
  • as a protease inhibitor in the preparation of brush-border membrane vesicles
  • for the processing of human synovial fluid, blood and eye sleep
  • to prevent non-specific degradation of cartilages

Biochem/physiol Actions

Benzamidine HCl is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases. A concentration of approximately 1 mM is used for general protease inhibition. To inhibit proteases from yeast, a range of 0.5 to 4.0 mM is used and it is for the most part interchangeable with pepstatin A.

In addition to being a strong competitive inhibitor of trypsin, benzamidine HCl has been also shown to be a strong competitive inhibitor of thrombin and plasmin. It was also found to be as effective as aprotinin in the prevention of glucagon degradation in human plasma.
Peptidase inhibitor.

Preparation Note

Benzamidine HCl is soluble in water and alcohol. Solubility testing in water at a concentration of 50 mg/ml yields a clear solution with heating. Benzamidine HCl is sensitive to oxidation. It is recommended to prepare solutions fresh each time in degassed water prior to use. However, frozen aliquots stored under inert gas, to exclude air, may be stable for a short time. Insufficient information is available to assess the shelf-life of a frozen solution.

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This Item
M6500B3650434760
assay

≥99%

assay

≥98% (titration)

assay

≥99% (TLC)

assay

99%

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

form

powder

form

powder or crystals

form

powder

form

beads

solubility

water: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

solubility

water: 50 mg/mL, clear, colorless to very faintly yellow

solubility

water: 50 mg/mL, clear, colorless to light yellow

solubility

water: soluble

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-


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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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