This product is a crude bile extract from ox gall bladder. B3883 is soluble in water at 50 mg per mL resulting in a turbid dark brown to green suspension. The natural concentration is 1 gram of solid material with 11.5 grams of water. We do not have more detailed information about using it in preparing a blank matrix.
B3883
Bile bovine
dried, unfractionated
Synonym(s):
Ox gall powder
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| Size/SKU | Availability | Price |
|---|---|---|
25 g | Please contact Customer Service for Availability | PLN 269.00 |
100 g | Please contact Customer Service for Availability | PLN 796.00 |
500 g | Please contact Customer Service for Availability | PLN 1,860.00 |
About This Item
sterility
non-sterile
Quality Segment
form
dried, unfractionated
color
yellow-brown
solubility
water: soluble 50 mg/mL
application(s)
microbiology
General description
Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.
Application
Bile bovine has been used:
- in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition
- as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology
- as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583
Biochem/physiol Actions
Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.
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This Item | |||
|---|---|---|---|
| form dried, unfractionated | form powder | form powder | form powder |
| application(s) microbiology | application(s) - | application(s) microbiology | application(s) microbiology |
| sterility non-sterile | sterility - | sterility non-sterile | sterility non-sterile |
| solubility water: soluble 50 mg/mL | solubility - | solubility distilled water: freely soluble | solubility H2O: 0.1 g/mL, clear, faintly yellow |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| color yellow-brown | color - | color - | color - |
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hi, I am trying to prepare blank bile matrix using this bovine bile powder. Do you have any recommendation of mixing ratio? Thank you
1 answer-
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WHAT IS THE PROPER TEMPERATURE FOR STORAGE?
1 answer-
Item B3883 can be stored at room temperature.
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Can you tell us the exact bile salt concentration?
1 answer-
This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however other components such as glycocholic and taurocholic acid are also present.
Helpful?
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I need to prepare bile salt (10 mM). May I know the content of Bile in this product? Or the molecular weight of this products? Thanks.
1 answer-
This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however, other components such as glycocholic and taurocholic acid are also present.
Helpful?
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