RADPCMAG-82K
MILLIPLEX® Rat Adipocyte Panel - Metabolism Assay
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Recommended Products
species reactivity
rat
Quality Level
manufacturer/tradename
Milliplex®
assay range
accuracy: 94-121%
sensitivity: 0.1-25 pg/mL
(MinDC+2SD)
standard curve range: 0.7-3,000 pg/mL
(IL-1β, TNFα)
standard curve range: 12-50,000 pg/mL
(PAI-1)
standard curve range: 122-500,000 pg/mL
(Adiponectin)
standard curve range: 15-60,000 pg/mL
(IL-6, Leptin)
standard curve range: 9.8-40,000 pg/mL
(MCP-1)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
Related Categories
General description
In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Also, its anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack.
The MILLIPLEX® Rat Adipocyte Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
The MILLIPLEX® Rat Adipocyte Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
The MILLIPLEX® MAP Rat Adipocyte Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα.
Specificity
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Application
- Analytes: Adiponectin, IL-1β, IL-6, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
- Recommended Sample Type: Rat cell/tissue culture supernatant or lysates.
- Recommended Sample Dilution: 25 μL per well of cell/Tissue culture sample. Samples may require a dilution with an appropriate control medium.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Endocrine; Obesity; Metabolic Disease
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
Everything you need in a single kit.
Components
Rat Adipokine Standard, 1 vial, lyophilized
Rat Adipokine Quality Control 1, 1 vial, lyophilized
Rat Adipokine Quality Control 2, 1 vial, lyophilized
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Adipocyte Detection Antibodies, 1 bottle, 5.5 mL
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
Rat Adipokine Quality Control 1, 1 vial, lyophilized
Rat Adipokine Quality Control 2, 1 vial, lyophilized
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Adipocyte Detection Antibodies, 1 bottle, 5.5 mL
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Other Notes
Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hamidah Abu Bakar et al.
Cardiovascular research, 113(8), 962-972 (2017-04-04)
Electrical field stimulation (EFS) elicits robust sensory neurogenic relaxation responses in the rat isolated mesenteric arterial bed but these responses are absent or difficult to demonstrate in isolated arteries. We believe that this mismatch is due to the absence of
Monika Kubacka et al.
Pharmaceuticals (Basel, Switzerland), 14(5) (2021-06-03)
Quinazoline α1-adrenoceptors antagonists have been shown to exert moderately favorable effects on the metabolic profile in hypertensive patients. However, based on AntiHypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial (ALLHAT) results, they are no longer recommended as a first
Irene Bretón Lesmes et al.
Nutricion hospitalaria, 38(Spec No1), 8-14 (2021-02-03)
Nutritional assessment and treatment, necessary for the prevention and treatment of disease-related malnutrition, should be carried out by a multidisciplinary team where each member has well-defined skills and functions, and mechanisms are established to allow adequate coordination, both in the
Salvador Peiró Moreno et al.
Nutricion hospitalaria, 37(6), 1246-1280 (2020-10-16)
Background: the legislation currently regulating the inclusion of Food for Special Medical Purposes (FSMP) among the benefits of the National Health System (SNS) was made approximately 15 years ago. The evolution of the technical characteristics of the FSMP justifies an
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Multiplex immunoassays save time and sample volume in metabolic syndrome research, providing comprehensive insights into related conditions.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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