MABS514

Anti-Keap1 Antibody, clone 144

clone 144, from rat

• Synonym(s): Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 144, monoclonal
• Application: FACS, ICC, WB
• Citations: 8

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 144, monoclonal
• species reactivity: human, mouse
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001103775
• UniProt accession no.: Q9Z2X8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KEAP1(9817)

Description

General description

KEAP1 is an important adapter protein important in regulating the transcription factor NFE2L2/NRF2. Nuclear factor erythroid-2 related factor 2 (NRF2) is a redox-sensitive transcription factor that positively regulates the expression of genes encoding antioxidants, xenobiotic detoxification enzymes, and drug efflux pumps, and confers cytoprotection against oxidative stress and xenobiotics in normal cells. KEAP1 negatively regulates NRF2 activity by targeting it to proteasomal degradation. KEAP1 acts as a substrate adapter protein for the E3 ligase ubiquitin protein complex formed by CUL3 and RBX1 proteins which mediate the ubiquitination and proteasome destruction of NRF2. KEAP1 also targets PGAM5 which is a central mediator of programmed necrosis induced by TNF and reactive oxygen species, so again KEAP1 plays an important role ultimately in cell survival and adaptation to its microenvironment. KEAP1 is widely expressed in many tissues, most strongly in muscle tissues and appears to shuttle from the cytoplasm to the nucleus depending upon the cellular context.

~69 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Recombinant protein corresponding to mouse Keap1.

Application

Flow Cytometry Analysis: 4.0 µg of this antibody from a representative lot detected Keap1 in 1X10E6 NIH/3T3 cells.
Western Blotting Analysis: A representative lot detected Keap1 in extracts from mouse embreyonic fibroblasts (MEF′s) (Watai, Y., et al. (2007). Genes to Cells. 12:1163-1178).
Western Blotting Analysis: A representative lot detected Keap1 in transfected HeLa cells (Tsuchiya, Y., et al. (2011). Mole. and Cell Biol. 31(22):4500-4512).
Immunocytochemistry Analysis: A representative lot detected Keap1 in mouse embreyonic fibroblasts (MEF′s) cells (Watai, Y., et al. (2007). Genes to Cells. 12:1163-1178).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-Keap1 Antibody, clone 144 is validated for use in Western Blotting and Flow Cytometry and Immunocytochemistry for the detection of Keap1.

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Keap1 in 10 µg of Raji cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable