MABF2811

Anti-Hemagglutinin Antibody, Influenza A virus H5N8 Antibody, clone 7H6C

• Synonym(s): HA-H5N8
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 7H6C, monoclonal
• Application: IF, WB, affinity binding assay
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7H6C, monoclonal
• mol wt: calculated mol wt 64.61 kDa, observed mol wt ~60 kDa
• purified by: using protein G
• species reactivity: virus
• packaging: antibody small pack of 100 μL
• technique(s): affinity binding assay: suitable, immunofluorescence: suitable, western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal half
• Protein ID accession no.: N/A
• UniProt accession no.: A0A096XG14, A0A0R9BPQ0, A0A0U2GP68
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: vaccinia virus ... HA(3654615)

Description

General description

Hemagglutinin (UniProt: A0A096XG14/ A0A0R9BPQ0; also known as HA) is encoded by the HA gene (Gene ID: 3654615) in avian influenza A virus of H5N8 strain. H5N8 strain is a highly pathogenic avian influenza virus. Hemagglutinin (HA), a surface glycoprotein of influenza viruses, is a major target for detection of the influenza virus subtype in the infected samples. HA protrudes from the outside of the virus particles in the form of a trimer. It is a single-pass type I membrane protein with a single transmembrane domain that is localized in amino acids 532-554. HA is cleaved into two chains known as HA1 and HA2 by the action of TMPRSS2 or a furin-like protease of host cells. The cleavage site is localized between amino acids 345 and 346. HA binds to the sialic acid-containing receptors on the cell surface that brings about the attachment of virus to the host cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. It can bind to animal red blood cells and cause a coagulation reaction (hemagglutination). Clone 7H6C binds to HA protein of H5N1 and H5N8, but not to HA proteins of H1N1, H3N2, and H7N9, indicating its specificity for H5-subtypes. (Ref.: Yu-Chen Cheng, Y-C., and Chang, S-C. (2020). Appl. Microbiol. Biotechnol. 105(1); 235-245).

Immunogen

His-tagged recombinant HA1 or HA2 proteins of Avian Influenza virus, H5N8 strain, expressed in E.coli.

Application

Quality Control Applications

Evaluated by Western Blotting with full-length, recombinant Hemagglutinin HA from the H5N8 strain of Avian influenza A virus.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected full-length, recombinant Hemagglutinin HA from the H5N8 strain of Avian influenza A virus, but not the recombinant Hemagglutinin HA2.

Tested applications

Inhibition: A representative lot inhibited Hemagglutinin activity in Influenza A virus H5N8 in (Cheng, Y-C. and Chang, SC. (2021). Appl Microbiol Biotechnol. 105(1); 235-245).

Electron Microscopy: 45 g/mL from a representative lot detected Hemagglutinin, Influenza A virus H5N8 in H5/H6/H7 trivalent VLPs. (Data provided by Dr. Hui-Wen Chen (Department of Veterinary Medicine, National Taiwan University).

Immunofluorescence Analysis: 4.5 µg/mL from a representative lot detected Hemagglutinin, Influenza A virus H5N8 in Recombinant baculovirus-infected Sf21 cells (Data provided by Dr. Hui-Wen Chen (Department of Veterinary Medicine, National Taiwan University).

Western Blotting Analysis: A representative lot detected Hemagglutinin, Influenza A virus H5N8 in Western Blotting applications (Cheng, Y-C., and Chang, SC. (2021). Appl Microbiol Biotechnol. 105(1); 235-245).

Affinity Binding Assay: A representative lot detected Hemagglutinin, Influenza A virus H5N8 in Affinity Binding applications (Cheng, Y-C., and Chang, SC. (2021). Appl Microbiol Biotechnol. 105(1;235-245).

ELISA Analysis: A representative lot detected Hemagglutinin, Influenza A virus H5N8 in ELISA applications (Cheng, Y-C., and Chang, SC. (2021). Appl Microbiol Biotechnol. 105(1); 235-245).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 7H6C is a mouse monoclonal antibody that detects hemagglutinin of Avian Influenza virus, H5N8 strain. It targets an epitope within 24 amino acids from the N-terminal half.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable