MABF151D

Anti-mouse IFN gamma, FITC, clone XMG1.2 Antibody

clone XMG1.2, from rat, FITC conjugate

• Synonym(s): Interferon gamma (IFN-gamma), IFN gamma
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: FITC conjugate
• Clone: XMG1.2, monoclonal
• Application: FACS
• Citations: -

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: XMG1.2, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• UniProt accession no.: P01580
• target post-translational modification: unmodified
• Gene Information: mouse ... Ifng(15978)

Description

General description

The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells, which allow the cytokine to modulate innate immunity, when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein, e.g. by flow cytometry, as well as for quantitative analysis of the secreted protein by ELISA, when paired with an appropriate secondary antibody. This clone is also widely used for neutralization of the functional activity of IFN-g in a variety of assays.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This rat monoclonal Anti-mouse IFN gamma Antibody, FITC, clone XMG1.2, Cat. No. MABF1517 is validated for use in Flow Cytometry, for the detection of IFN gamma.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected IFN gamma in one million C57Bl/6 splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg