MABE972

Anti-PRDM5 Antibody

clone 57-20, from mouse

• Synonym(s): PRDM5, PR domain zinc finger protein 5, PR domain-containing protein 5
• UNSPSC Code: 12352203
• NACRES: NA.43
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 57-20, monoclonal
• Application: IP, WB
• Citations: 2

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 57-20, monoclonal
• species reactivity: mouse
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_061169
• UniProt accession no.: Q9NQX1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Prdm5(70779)

Description

General description

PR domain zinc finger protein 5 (UniProt Q9NQX1) is encoded by the PRDM1 gene (also known as BCS2, PFM2) in human (Gene ID 11107). PRDM proteins constitute a family of transcriptional regulators that are characterized by the presence of variable numbers of zinc finger domains and an N-terminal PR domain. PRDM5 can negatively regulate gene transcriptions by recruiting the G9a histone methyltransferase and histone deacetylases. PRDM5 can promote transcription by targeting enhancer-like elements or recruiting RNA polymerase II binding. PRDM5 is highly expressed in murine embryonic stem cells (mESCs) and associates with complexes involved in chromatin organization, such as Ctcf, Smc1 (cohesin), and TFIIIC, which indicates their involvement in chromatin organization. PRDM5 is often found silenced in human breast, ovarian, liver, and cervical cancers.

~73 kDa observed. 73.1 kDa (isoform 1, calculated), 69.9 kDa (isoform 2, calculated), 12.9 kDa (isoform 3, calculated), 58.1 kDa (isoform 4, calculated).

Immunogen

GST-tagged recombinant protein corresponding to human PRDM5 near the N-terminus.

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated PRDM5 in mouse embryonic fibroblasts (Data courtesy of Dr. Anders H. Lund, University of Copenhagen, Denmark).
Immunoprecipitation: A representative lot of this antibody immunoprecipitated PRDMS E9 and E10 intrahepatic cholangiocarcinoma (ICC) cells. (Seehawer, M., et al. (2018). Nature 562(7725); 69-75).

This Anti-PRDM5 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of PRDM5 .

Biochem/physiol Actions

Expected to react with all four spliced isoforms

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in mouse ESC lysate.

Western Blotting Analysis: 8.0 µg/mL of this antibody detected PRDM5 in 10 µg of mouse ESC lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable