MABD116

Anti-Dux4 Antibody, clone 9A12

clone 9A12, from mouse

• Synonym(s): double homeobox protein 4, Double homeobox protein 10, Double homeobox protein 4/10, Dux-4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 9A12, monoclonal
• Application: EMSA, ICC, IP, WB
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9A12, monoclonal
• species reactivity: human
• technique(s): electrophoretic mobility shift assay: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_149418
• UniProt accession no.: Q9UBX2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DUX4(100288687)

Description

General description

Dux4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in Dux4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Ectopic expression of Dux4 in skeletal muscle activates the expression of stem cell and germline genes, and, when overexpressed in somatic cells, Dux4 can ultimately lead to cell death.

~58 kDa observed

Immunogen

Epitope: domain of: C-Terminal region

recombinant protein corresponding to the domain of: C-Terminal region of Human Dux4.

Application

Detect Dux4 using this mouse monoclonal antibody, Anti-Dux4 Antibody, clone 9A12 validated for use in western blotting, ICC, Electrophoretic Mobility Shift Assay & IP.

Research Category
Stem Cell Research

Stem Cell Research

Research Sub Category
Muscle Physiology

Developmental Signaling

This antibody has been shown to work in immunocytochemistry applications (Dixit et al. 2007). This antibody has been shown to work in electrophoretic mobility shift assays (Dixit et al. 2007). This antibody has been shown to work in immunoprecipitation applications (Anseau et al., 2009).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human skeletal muscle tissue lysate.

Evaluated by Western Blot in a human skeletal muscle tissue lysate. Western Blot Analysis: A 1:250 dilution of this antibody detected Dux4 in a human skeletal muscle tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable