MABC586

Anti-Sulf-2 Antibody, clone 8G1

clone 8G1, from mouse

• Synonym(s): Extracellular sulfatase Sulf-2, hSulf-2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 8G1, monoclonal
• Application: ICC, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8G1, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001155313
• UniProt accession no.: Q8IWU5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SULF2(55959)

Description

General description

Sulf-2 is an endoplasmic reticulum enzyme that is involved in sulfur metabolism which is important in bone, cartilage, and skeletal development. Sulf-2 is also important in neurological situations including myelination and glial cell differentiation and GDNF activity. Sulf-2 activity is also found in the kidney and important in glomerular basement membrane development and glomerular function. Finally, Sulf-2 is involved in heparan sulfate metabolism. Sulf-2 removes sulfate moieties on heparan sulfate proteoglycans which in turn modulate the heparin binding growth factor signaling activity of the cell. Sulf-2 also impacts extracellular matrix interactions and it is important during development and growth, as well as Wnt signaling and VEGF production by endothelial cells during vascularization. In disease, Sulf-2 expression in cancer is upregulated and inhibition of Sulf-2 limits cancer progression and showed increased apoptosis within tumors, thus Sulf-2 activation is being examined as a potential anti-cancer therapy.

~125 & 75 kDa observed.
Sulf-2 is synthesized as a ~125 kDa pre-protein and is subsequently cleaved into ~75 kDa and ~50 kDa fragments, which are held together by disulfide bonds in the mature protein.

Immunogen

Recombinant protein corresponding to Human Sulf-2.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Tumor Markers

This Anti-Sulf-2 Antibody, clone 8G1 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sulf-2.

Western Blot Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cell lysates.

Immunocytochemistry Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cells, but not in HEK293 cells transfected with an empty vector or Sulf-1.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in MCF-7 conditioned media.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sulf-2 in 10 µg of MCF-7 conditioned media.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1