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antibody form
purified antibody
clone
4D6 (-3H10-D6)
monoclonal
purified by
using protein G
species reactivity
human, mouse, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
General description
His-tagged recombinant protein fragment corresponding to the N-terminal region of human Cereblon.
Specificity
Clone 4D6 (-3H10-D6) is a mouse monoclonal antibody that detects human Cereblon. It targets an epitope within the N-terminal domain.
Application
Western Blotting Analysis: 20 ug from a representative lot detected CRBN in MOLT4 wild type cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CRBN in MOLT4 wild type cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna Austria).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CRBN in MOLT4 wild type cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna Austria).
Quality
Evaluated by Western Blotting in MOLT-4 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Cereblon.(CRBN) in MOLT-4 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Cereblon.(CRBN) in MOLT-4 cell lysate.
Physical properties
~50 kDa observed; 50.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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