MABC1037

Anti-Sp100 Antibody, clone 2H1.1

clone 2H1.1, from mouse

• Synonym(s): Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, Speckled 100 kDa
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 2H1.1, monoclonal
• Application: ICC, WB
• Citations: 2

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2H1.1, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_003104
• UniProt accession no.: P23497
• target post-translational modification: unmodified
• Gene Information: human ... SP100(6672)

Description

General description

Nuclear autoantigen Sp-100 (UniProt P23497; also known as Nuclear dot-associated Sp100 protein, Speckled 100 kDa) is encoded by the SP100 gene (Gene ID 6672) in human. The SP100 family genes, including SP100, SP110, SP140 and SP140L, encode highly similar proteins mainly expressed in leukocytes. Interferons (IFNs) and other viral infection-related stimuli induce SP100 family gene expression and mutations of SP100 genes are linked to immune diseases and cancers. SP100 colocalizes with promyelocytic leukemia (PML) protein in nuclear bodies (NBs), also known as nuclear domain 10 (ND10), that function to regulate gene transcription via chromatin-based mechanisms and thereby mediate a variety of cellular functions including senescence, apoptosis, protein degradation, telemere stasbility, and defense against viral infections.

~100/78 kDa observed. 100.3 kDa (isoform Sp100-HMG/SP100HMG/SpAlt-HMG), 53.64 kDa (isoform Sp100-A/SP100A/SP100), 78.43 kDa (isoform Sp100-B/SP100B/SpAlt-212), 101.4 kDa (isoform Sp100-C/SP100C), 52.98 kDa (isoform SpAlt-C), 50.46 kDa (isoform 6), and 49.90 kDa (isoform 7) calculated (with Met1 removed). Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: Internal (N-terminal half).

GST-tagged recombinant human Sp100 internal fragment.

Application

Anti-Sp100 Antibody, clone 2H1.1 is an antibody against Sp100 for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Sp100 nuclear foci in HeLa cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Chromatin Biology

Biochem/physiol Actions

Clone 2H1.1 targeted epitope region is present in all seven human Sp100 spliced isoforms reported by UniProt (P23497).

Physical form

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in HepG2 nuclear extract.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Sp100 in 10 µg of HepG2 nuclear extract.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable