MAB5428

Anti-Retinal Pigment Epithelium 65 Antibody

clone 401.8B11.3D9, Chemicon^®, from mouse

• Synonym(s): RPE65
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 401.8B11.3D9, monoclonal
• Application: ELISA, IHC, IP, WB
• Citations: 42

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 401.8B11.3D9, monoclonal
• species reactivity: bovine, human, Xenopus, mouse
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_000329.2
• UniProt accession no.: Q16518
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... RPE65(6121)

Description

General description

~ 65 kDa

Immunogen

Bovine RPE microsomal membranes.

Application

Anti-Retinal Pigment Epithelium 65 Antibody detects level of Retinal Pigment Epithelium 65 & has been published & validated for use in ELISA, IH, IP & WB.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

Western blot: 1:5,000-1:20,000 on bovine RPE membranes using ECL. Suggested dilution buffer is TBS containing 10% calf serum, 0.25% T-20, 1M D-glucose with 10% glycerol. Suggested blocking buffer is TBS containing 2% BSA and 0.5% Tween 20. Preferred gel percentage is 10%.

Immunohistochemistry on frozen tissue sections: 1:250-1:500

Immunoprecipitation: 20 μg of antibody in a reaction volume of 500 μL.

Immunoaffinity purification

ELISA

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with Retinal Pigment Epithelium 65 (RPE65). On bovine RPE membranes the antibody recognizes a protein with a molecular weight of ~65 kDa.

Physical form

Format: Purified

Protein A purified

Purified immunoglobulin. Liquid in PBS. Contains no preservative.

Preparation Note

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Eye

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• Storage Class: 12 - Non Combustible Liquids