MAB3152

Anti-Cardiac Troponin I Antibody, a.a. 87-91, clone 8E10

clone 8E10, Chemicon^®, from mouse

• Synonym(s): cTn1, Cardiac Troponin I
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 8E10, monoclonal
• Application: ELISA, WB
• Citations: 3

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• clone: 8E10, monoclonal
• species reactivity: canine, rabbit, feline, human, bovine, pig, goat
• should not react with: rat, mouse, fish
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000363.4
• UniProt accession no.: P19429
• shipped in: wet ice
• target post-translational modification: unmodified

Description

Immunogen

Epitope: a.a. 87-91

Free human cardiac troponin 1.

Application

Detect Cardiac Troponin I using this Anti-Cardiac Troponin I Antibody, a.a. 87-91, clone 8E10 validated for use in ELISA & WB.

Research Category
Metabolism

Research Sub Category
Muscle Physiology

Western blotting: identifies two bands 14kDa and 18kDa which appear to be breakdown products of full length cTn1 (Morjana N. 1998, Degradation of human cardiac troponin I after myocardial infarction. Biotechnol Appl. Biochem. 28:105-11).

EIA: MAB3152 has been used successfully in ELISA and immunoaffinity purification. Its affinity constant is 8x10(10). It can be used with MAB3150 in sandwhich ELISA as the coating antibody.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

MAB3152 reacts equally with free cardiac troponin 1 (cTn1) and cTn1 forming complexes with other troponin components. It recognizes epitopes that are not perturbed or sterically shielded by other troponin components. Not affected by heparin, phosphorylation, oxidation and complex formation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. There is no cross-reaction with skeletal muscle troponin 1.

Physical form

Format: Purified

Liquid in PBS (pH 7.4) with 0.1% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable