AP501B

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed

Chemicon^®, from goat

• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: biotin conjugate
• Clone: polyclonal
• Application: ELISA, IF
• Citations: -

Properties

• biological source: goat
• Quality Segment: 100
• conjugate: biotin conjugate
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunofluorescence: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Immunogen

Epitope: Mouse IgM, IgG, IgA

Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.

Application

Detect Mouse IgM+IgG+IgA (H+L) using this Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed validated for use in ELISA & IF.

Immunofluorescence: ≤1 μg/10E6 cells
ELISA: 1:5,000-1:20,000

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Biochem/physiol Actions

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.

The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Physical form

Affinity Chromatography

Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.

Preparation Note

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 12 - Non Combustible Liquids