ABT129

Anti-Calponin-1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Calponin-1, Basic calponin, Calponin H1, smooth muscle
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IHC, WB
• Citations: 4

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): sheep (based on 100% sequence homology), bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_001290
• UniProt accession no.: P51911
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CNN1(1264)

Description

General description

Calponin 1 is a 34 kDa protein that is predominantly found in smooth muscle tissue. It is a high-affinity actin-binding protein, but also interacts with tropomyosin, and calmodulin to modulate muscle contraction. When bound to F-actin, it inhibits the activity of the actin-activated myosin ATPase. Calponin 1 is regulated by reversible phosphorylation involving PKC and CAMKII kinases, and type 2A phosphatases. In non-muscle cells, calponin 1 may play a role in cell migration, motility, and proliferation.

~33 kDa observed

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human Calponin-1.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a reprsesentative lot detected Calponin-1 in human seminal vesicle tissue.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Calponin-1 in smooth muscle cells from human seminal vesicle tissue.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Calponin-1 Antibody is validated for use in Western Blotting, IHC(P), Immunofluorescence for the detection of Calponin-1.

Biochem/physiol Actions

Other homologies: Rat (92% sequence homology) and Mouse (83% sequence homology).

This antibody recognizes the C-terminus of Calponin-1.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human aortic smooth muscle tissue

Evaluated by Western Blot in human aortic smooth muscle tissue.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Calponin-1 in human aortic smooth muscle tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-589

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable