ABN58

Anti-phospho MAP1B (Thr1265) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Microtubule-associated protein 1B, MAP-1B, MAP1(X), MAP1.2, MAP1 light chain LC1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: rat
• Application: ICC, IHC, WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• species reactivity (predicted by homology): mouse (immunogen homology)
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• UniProt accession no.: P14873
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr1265)
• Gene Information: mouse ... Map1B(17755) ; rat ... Map1B(29456)

Description

General description

Microtubule-associated protein 1B (MAP1B) is neuron-specific and is thought to play a part in controlling the neuronal cytoskeleton. MAP1B is made up of heavy and light chains and is involved in the microtubule and actin filament association. MAP1B may have an important role in axon formation by supporting microtubule dynamics and controlling cytoskeletal arrangement. MAP1B has also been associated with Netrin 1 signaling.

~300 kDa observed

Immunogen

Epitope: Phosphorylated Thr1265

KLH-conjugated linear peptide corresponding to mouse MAP1B phosphorylated at Thr1265.

Application

Anti-phospho MAP1B (Thr1265) Antibody is an antibody against phospho MAP1B (Thr1265) for use in WB, IH & IC.

Immunohistochemistry Analysis: 1:600 dilution from a representative lot detected MAP1B in embryonic (E12) rat spinal cord tissue. (Image courtesy of Dr. Phillip R. Gordon-Weeks, King′s College London.)

Immunofluorescence Analysis: A representative lot was used by an independent laboratory in IF. (Trivedi, N., et al. (2005). Journal of Cell Science. 118:993-1005.)

Biochem/physiol Actions

Other homologies: Human (91% sequence homology).

This antibody recognizes MAP1B phosphorylated at Thr1265.

Analysis Note

Control
S1 rat brain tissue lysate

Evaluated by Western Blot in S1 rat brain tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected MAP1B on 10 µg of S1 rat brain tissue lysate.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2