ABN433

Anti-Corticotropin-Releasing Factor Receptor 2 Antibody

1.0 mg/mL, from rabbit

• Synonym(s): Corticotropin-releasing factor receptor 2, CRF-R-2, CRF-R2, CRFR-2, Corticotropin-Releasing Factor Receptor 2, Corticotropin-releasing hormone receptor 2, CRH-R-2, CRH-R2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): monkey (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), hamster (based on 100% sequence homology), primate (based on 100% sequence homology)
• concentration: 1.0 mg/mL
• technique(s): immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_001189404
• UniProt accession no.: Q13324
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CRHR2(1395)

Description

General description

Corticotropin-Releasing Factor Receptor 2 (CRFR2) is a G-protein coupled receptor for CRH (Corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Ligand binding activates adenylate cyclase leading to an increase in cAMP levels and subsequent gene activation. CRFR2 binds Urocortin (UCN) with the highest affinity. CRFR2 is localized to the plasma membrane, and CRFR2 has numerous isoforms but their functional importance remains to be discovered. Latest research is focused upon antagonists of the receptors for CRF. Astressin2-B, which is specific for CRFR2 and Astressin B which binds both CRFR1 and CRFR2 are two recent peptide antagonists. The research hopes to develop treatments for many CRF-dependent diseases in the periphery such as irritable bowel syndrome and even perhaps various social behaviors such as anxiety and anti-social activities.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human Corticotropin-Releasing Factor Receptor 2 near the N-terminal.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Corticotropin-Releasing Factor Receptor 2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Corticotropin-Releasing Factor Receptor 2.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Corticotropin-Releasing Factor Receptor 2 in 10 µg of human brain tissue lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Corticotropin-Releasing Factor Receptor 2 in human colon and Bowman′s capsule epithelial cells.

Quality

Evaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Corticotropin-Releasing Factor Receptor 2 in 10 µg of human colon tissue lysate.

Target description

~49 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing from ~45-65 kDa

Linkage

Replaces: AB9420

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable