ABF1065

Anti-SARS-CoV-2 spike protein S1 domain

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA, Neutral, WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified antibody, serum
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: calculated mol wt 139.13 kDa, observed mol wt ~38 and 170 kDa
• purified by: (Unpurified)
• species reactivity: virus
• packaging: antibody small pack of 100 μL
• technique(s): ELISA: suitable, neutralization: suitable, western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: YP_009724390
• UniProt accession no.: P0DTC2
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: vaccinia virus ... s>S(43740568)

Description

General description

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. This polyclonal antibody binds and neutralizes the SARS-CoV-2 variants including the B.1.1.7; B.1.429; P.1; and B.1.351 at different levels. (Ref.: Ravichandran, S., et al. (2020). Sci Transl Med. 12(550):eabc3539).

Immunogen

His-tagged recombinant fragment corresponding to the S1 domain (aa 16-685) from the extracellular region of SARS-CoV-2 spike protein.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant S1 domain and the extracellular domain of SARS-CoV-2 spike protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant S1 domain and the complete extracellular domain of SARS-CoV-2 spike protein.

Tested Applications

Enzyme Immunoassay Analysis: A representative lot detected SARS-CoV-2 spike protein S1 domain in ELISA applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).

Neutralizing: A representative lot neutralized SARS-CoV-2 spike protein S1 domain in Neutralizing applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV-2 spike protein S1 domain, Cat. No. ABF1065, is a rabbit polyclonal antibody that detects the S1 domain of SARS-CoV-2 spike protein and is tested for use in ELISA, Neutralizing, and Western Blotting.

Biochem/physiol Actions

This rabbit polyclonal antibody detects the S1 domain of SARS-CoV-2 spike protein.

Physical form

Rabbit polyclonal antibody in serum without preservatives.

Preparation Note

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable