ABE1402

Anti-LHX2 Antibody

serum, from rabbit

• Synonym(s): LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein, LHX2
• UNSPSC Code: 12352203
• NACRES: NA.43
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ChIP, IF, WB
• Citations: 9

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human
• technique(s): ChIP: suitable (ChIP-seq), immunofluorescence: suitable, western blot: suitable
• NCBI accession no.: NP_034840
• UniProt accession no.: Q9Z0S2
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Lhx2(16870)

Description

General description

Target molecular weight ~ 48 kDa observed. 44.42 kDa kDa calculated. Uncharacterized bands may be observed in some lysate(s).

LIM/homeobox protein Lhx2 (UniProt Q9Z0S2; also known as Homeobox protein LH-2, LIM homeo box protein 2, Lim2) is encoded by the Lhx2 gene (Gene ID 16870) in murine species. Lhx2 is a transcription factor that plays an important role in controlling the behavior of progenitor cells involved in the development of the skin hair cycling, eye, forebrain, limb, hematopoietic, olfactory, and pituitary systems. Lhx2 is shown to regulate a significant number of hair follicle stem cell (HF-SC) signature genes, as well as cytoskeletal and adhesion molecules within the niche where HF-SCs reside as a single layer of polarized, quiescent epithelial cells. Lhx2 deficiency leads to baldness, because the niche can no longer anchor its hair and maintain the proper behavior of its SCs as a result of greatly perturbed HF-SCs polarization and niche architecture.

Immunogen

GST/His tagged, full-length recombinant mouse LHX2.

Application

Tested Applications

Western Blotting Analysis: A 1:4,000 dilution from a representative lot detected LHX2 in lysate from doxycycline treated murine K14rtTA-LHX2-TRE keratinocytes and human brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected LHX2 in mouse embryo tissue sections.

Immunofluorescence Analysis: A representative lot of this antibody detected LHX2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University; Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).

ChIP seq Analysis; A representative lot of this antibody detected LHX2 by ChIP-seq analysis (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

This polyclonal antibody specifically detects LHX2.

Immunofluorescence Analysis: A representative lot detected Lhx2 immunoreactivity by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed backskin cryosections from wild-type, but not Lhx2 knockout, mice at varies developmental stages, including E16.5, P0, P30, and the second telogen (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions by a genome-wide ChIP-seq analysis using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Immunofluorescence Analysis: A representative lot (1:1,000 dilution) detected Lhx2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University ).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Physical form

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Preparation Note

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected LHX2 in mouse brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable