AB3832

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific

Chemicon^®, from rabbit

• Synonym(s): Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: monkey, human, mouse, rat
• Application: IHC, WB
• Citations: 4

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: monkey, human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NM_004252.2
• UniProt accession no.: O14745
• shipped in: dry ice
• target post-translational modification: phosphorylation (pThr567)
• Gene Information: human ... SLC9A3R1(9368)

Description

General description

75KDa Moesin, and ~80kDa Ezrin & Radixin

Immunogen

Epitope: phospho-specific

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin

Application

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.

Immunohistochemistry (paraffin): 1:50

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.

Physical form

ImmunoAffinity Purified

Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.

Preparation Note

Maintain at -20°C for 12 months, do not aliquot.

Analysis Note

Control
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2