AB16982

Anti-Fas Antibody

CHEMICON^®, rabbit polyclonal

• Synonym(s): FasL
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: human, mouse, rat
• Application: ELISA, WB
• Citations: 10

Properties

• Product Name: Anti-Fas Ligand Antibody, Chemicon^®, from rabbit
• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, western blot: suitable
• NCBI accession no.: NM_000043.3, NM_152871.1, NM_152872.1, NM_152873.1, NM_152874.1, NM_152875.1, NM_152876.1, NM_152877.1
• UniProt accession no.: P25445
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... FAS(355)

Description

Immunogen

A 13 AA peptide sequence mapping near the C-terminus of human FAS ligand (Mita et al. 1994). This peptide is predicted to be extracellular. The peptide was synthesized with C-terminal Cysteine, coupled to KLH.

Application

Western Blotting: 1:1,000-1:5,000 Rat, mouse and human FASL have 278, 279, and 281 AA residues, respectively (calculated MW 32kDa). Western blot of the oocyte has detected a specific 31kDa band (Hakuno et al. 1996). A 40 kDa FASL has been detected in mouse T cells (Hahne 1995). An alternate detecting reagent, mFAS-Fc (Suda & Nagata 1994) also detected a cell surface protein of about 40 kDa.

ELISA: 0.5-1μg/mL

Optimal working dilutions must be determined by the end user.

Anti-Fas Ligand Antibody detects level of Fas Ligand & has been published & validated for use in ELISA & WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

Human FAS Ligand (FASL). The 13-mer human FASL sequence used for antibody production is 92% (12/13 AA) homologous with rat and mouse FASL. Based upon the sequence homology data, anti-human FASL will likely cross react with the mouse/rat FASL.

Physical form

Peptide affinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable