516360

Pepsin, Porcine Stomach Mucosa

Catalyzes the hydrolysis of aminoacyl-proline to an amino acid and proline. Inhibitors include aliphatic alcohols, pepstatin A, and pH 6.0.

• Synonym(s): Pepsin, Porcine Stomach Mucosa
• CAS Number: 9001-75-6
• EC Number: 3.4.23.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.25
• MDL number: MFCD00081840
• eCl@ss: 42010127
• Specific activity: ≥2000 units/mg protein

Properties

• Quality Segment: 100
• description: Merck USA index - 14, 7146
• form: lyophilized
• specific activity: ≥2000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• solubility: 10 mM HCl: 0.5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native pepsin from porcine stomach mucosa. Secreted by the mucosal lining of the stomach. Active under acidic conditions. Catalyzes the hydrolysis of aminoacyl-proline to an amino acid and proline. Inhibitors include aliphatic alcohols, pepstatin A, and pH >6.0.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Bergmeyer, H.V. 1984. Methods of Enzymatic Analysis (3rd ed., vol. 5) p. 232, Academic Press, New York.

One unit is defined as the amount of enzyme that will digest hemoglobin substrates to TCA-soluble products, measured as a change in absorbance at 280 nm of 0.001 per min at 37°C, pH 2.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable