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48-680MAG

Millipore

MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay

Synonym(s):

ERK Cell Signaling Assay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

species reactivity

human

Quality Level

200

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

storage temp.

2-8°C

Related Categories

General description

The Milliplex® MAP 9-plex Multi-Pathway Signaling Magnetic Bead kit, phosphoprotein, is used to detect changes in phosphorylated ERK/MAP kinase 1/2 (Thr185/Tyr187), Akt (Ser473), STAT3 (Ser727), JNK (Thr183/Tyr185), p70S6K (Thr412), NFkB (Ser536), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using the Luminex® xMAP® technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

Analyze a cell signaling panel using this kit. The analytes available for the cell signaling multiplex assay kit are: CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (Thr180/Tyr182), p70S6K (Thr412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473).
Research Category
Cell Signaling
Research Sub Category
Oncology
Used to detect/quantify:CREB (pS133)|ERK (pT185/pY187)|NFκB (pS536)|JNK (pT183/pY185)|p38 (Thr180/Tyr182)|p70S6K (Thr412)|STAT3 (pS727)|STAT5A/B (pY694/699)|Akt (pS473)

Storage and Stability

Recommended storage for kit components is 2 - 8°C

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sophie A Millar et al.
Journal of cellular physiology, 235(4), 3414-3424 (2019-09-25)
Some human observational studies have suggested an anti-inflammatory role of osteocalcin (OCN). An inflammatory protocol using interferon-γ and tumor necrosis factor-α (10 ng/ml) was employed to examine the acute (24 hr) and chronic (144 hr) effects of uncarboxylated OCN (ucOCN) in commercial, primary
Paul S Hafen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 125(5), 1447-1455 (2018-07-20)
The heat stress response is associated with several beneficial adaptations that promote cell health and survival. Specifically, in vitro and animal investigations suggest that repeated exposures to a mild heat stress (~40°C) elicit positive mitochondrial adaptations in skeletal muscle comparable
Christopher P Stanley et al.
Cardiovascular research, 107(4), 568-578 (2015-06-21)
The protective effects of cannabidiol (CBD) have been widely shown in preclinical models and have translated into medicines for the treatment of multiple sclerosis and epilepsy. However, the direct vascular effects of CBD in humans are unknown. Using wire myography
Paolo Carpineto et al.
Investigative ophthalmology & visual science, 57(15), 6895-6901 (2016-12-22)
To evaluate signal transduction and early apoptosis protein levels in subretinal fluid collected during scleral buckling surgery for macula-off rhegmatogenous retinal detachment (RRD). Our aim was to assess both their relation with RRD features and their influence on the posttreatment
William H Hind et al.
British journal of pharmacology, 173(5), 815-825 (2015-10-27)
In vivo and in vitro studies have demonstrated a protective effect of cannabidiol (CBD) in reducing infarct size in stroke models and against epithelial barrier damage in numerous disease models. We aimed to investigate whether CBD also affects blood-brain barrier
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