427211

Lactate Dehydrogenase, Porcine Heart

• Synonym(s): Lactate Dehydrogenase, Porcine Heart
• CAS Number: 9001-60-9
• EC Number: 1.1.1.27 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00131466
• Specific activity: ≥300 units/mg protein

Properties

• description: Merck USA index - 14, 5334
• Quality Segment: 100
• form: lyophilized
• specific activity: ≥300 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• impurities: ≤0.01% PK, MK, ≤0.03% GPT, GOT, ≤0.1% MDH
• pI: 4.6
• solubility: aqueous buffer: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.

Note: 1 KU = 1000 units

Preparation Note

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.

Other Notes

Kornberg, A. 1995. Methods Enzymol.1, 441

One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1