16-156

Protein A Agarose, Fast Flow

Protein A Agarose, Fast Flow suitable for medium and low-pressure chromatography, immunoprecipitation and antibody purification.

• Synonym(s): Protein A resin
• UNSPSC Code: 41116133
• NACRES: NA.56
• eCl@ss: 32160801

Properties

• form: liquid
• manufacturer/tradename: Upstate^®
• technique(s): affinity chromatography: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• shipped in: wet ice

Description

General description

Protein A is an immunoglobulin (Ig)-binding protein used to purify large amounts of IgG. It binds to the Fc part of the antibody at the CH₂–CH₃ interface. Protein A-agarose might be suitable for low-pressure antibody isolation.
Recombinant Protein A covalently coupled to highly cross-linked 6% agarose beads.

Binding capacity: 40mg human IgG/ml agarose

Application

Protein A Agarose, Fast Flow has been used in immunoprecipitation and chromatin immunoprecipitation (ChIP).

Physical form

sterile distilled water containing 0.01% thimerosal

Analysis Note

routinely evaluated in immunoprecipitation

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1