07-520

Anti-eNOS/NOS III Antibody, CT

Upstate^®, from rabbit

• Synonym(s): Constitutive NOS, Endothelial NOS, Endothelial NOS, Endothelial NOS, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 15

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, pig, guinea pig, rat, canine, human, bovine
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_000594
• UniProt accession no.: P29474
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Nos3(287024) ; dog ... Nos3(403784) ; guinea pig ... Nos3(100135577) ; human ... NOS3(4846) ; mouse ... Nos3(18127) ; pig ... Nos3(397557) ; rat ... Nos3(24600)

Description

General description

135 kDa

Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.

Application

Anti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.

Biochem/physiol Actions

Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.

Recognizes eNOS/NOS III, Mr ~135 kDa.

Physical form

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Analysis Note

Control
Platelets, placenta, liver and kidney.

Routinely evaluated by western blot in RIPA lysates from HUVEC cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1