07-108
Anti-Histone H4 Antibody
Upstate®, from rabbit
Synonym(s):
H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
Xenopus, mouse, avian, chicken, human, bovine
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
bovine ... H4C1(617905)
frog ... H4C1(549623)
human ... H4C1(8359)
mouse ... H4C1(326619)
General description
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Specificity
Broad species cross-reactivity expected.
Recognizes Histone H4, Mr 10 kDa.
Immunogen
Calf thymus histone H4.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-Histone H4 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Histone H4 also known as Histone H4.
Quality
Routinely evaluated by western blot on acid extracts of HeLa cells treated with sodium butyrate.
Western Blot Analysis:
0.1-0.5 μg/mL of this lot detected Histone H4 in acid extracts of HeLa cells treated with or without 5 mM sodium butyrate. This lot did not cross react with Histone H2A, Histone H2B or Histone H3.
Western Blot Analysis:
0.1-0.5 μg/mL of this lot detected Histone H4 in acid extracts of HeLa cells treated with or without 5 mM sodium butyrate. This lot did not cross react with Histone H2A, Histone H2B or Histone H3.
Target description
10 kDa
Physical form
Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% Sodium Azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Analysis Note
Control
HeLa cell extract.
HeLa cell extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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S L Gilbert et al.
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Microscopy studies have shown that XIST RNA colocalizes with the inactive X chromosome (Xi). However, the molecular basis for this colocalization is unknown. Here we provide two lines of evidence from chromatin immunoprecipitation experiments that XIST RNA physically associates with
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