06-680

Anti-Androgen Receptor Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ChIP, FACS, ICC, IHC, IP, WB
• Citations: 200

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human
• species reactivity (predicted by homology): bovine (based on 100% sequence homology), canine (based on 100% sequence homology), horse (based on 100% sequence homology), mouse (based on 100% sequence homology)
• technique(s): ChIP: suitable (ChIP-seq), flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_036634
• UniProt accession no.: P15207
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Ar(280675) ; dog ... Ar(403588) ; human ... AR(367) ; mouse ... Ar(11835) ; rat ... Ar(24208)

Description

General description

The androgen receptor (AR) is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The main function of the androgen receptor is as a DNA binding transcription factor which regulates gene expression. However the androgen receptor also has additional functions independent of DNA binding. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

~110 kDa observed

Immunogen

Epitope: This antibody recognizes the Modulation Region within the N-terminus of Androgen Receptor.

KLH-conjugated linear peptide corresponding to the N-terminus of rat Androgen Receptor.

Application

Anti-Androgen Receptor Antibody, PG-21 is a Rabbit Polyclonal Antibody for detection of Androgen Receptor also known as Dihydrotestosterone Receptor & has been tested in ICC, IHC, IP & WB.

Immunoprecipitation Analysis: 34 µL from a representative lot immunoprecipitated Androgen Receptor from 1000 µg of LNCaP cell lysate.

Flow Cytometry Analysis: A 1:300 dilution from a representative lot detected Androgen Receptor in 0.2X10E6 MCF-7 cells.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Li, X., et al. (2011). PLoS One. 6(9):e25040.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in embryonic rat tissue, neonatal rat testis tissue, and adult rat testis tissue (Nation, T., et al. (2010). J Pediatr Surg. 46(8): 1539-1543.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in MCF-7 cells stabily transfected with Androgen Receptor (Ghatge, R. P., et al. (2005). Breast Cancer Res. 7(6):R1036-1050.).

Chromatin Immunoprecipitaton-Sequence Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from LNCaP cell lysates under certain treated conditions (Zhao, J. C., et al. (2012). Genome Res. 22(2):322-331.).

Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Androgen Receptor in LNCaP cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Androgen Receptor in 10 µg of LNCaP cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable