05-807
Anti-phospho-CREB (Ser133) Antibody, clone 634-2
clone 634-2, Upstate®, from mouse
Synonym(s):
active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, transactivator protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
634-2, monoclonal
species reactivity
rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer133)
Gene Information
human ... CREB1(1385)
General description
CREB is a β ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.
Specificity
Broad species cross-reactivity expected, based on sequence homology.
Phospho-CREB (Ser133).
Immunogen
A proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.
Epitope: Ser133
Application
Beadlyte Specificity Assay:
1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence
Immunofluorescence / Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence.
1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence
Immunofluorescence / Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-phospho-CREB (Ser133) Antibody, clone 634-2 (mouse monoclonal antibody) validated in ICC, IF, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
Quality
Routinely evaluated by western blot on RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells.
Western Blot Analysis:
A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A).
Western Blot Analysis:
A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A).
Target description
43 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse IgG1κ in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20ºC.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Control
Human breast carcinoma, forskolin- and FGF-treated SK-N-MC cell extracts.
Human breast carcinoma, forskolin- and FGF-treated SK-N-MC cell extracts.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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