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05-321MG

Anti-Phosphotyrosine Antibody, clone 4G10®

clone 4G10®, Upstate®, from mouse

Synonym(s):

Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody

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Size/SKUAvailabilityPrice
1 mg

Estimated to ship onJune 05, 2026fromKuehne + Nagel Sp. z o.o.

PLN 21,170.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
4G10®, monoclonal
Technique(s):
immunoprecipitation (IP): suitable, western blot: suitable
Application:
IP, WB
Citations:
6

PLN 21,170.00


Estimated to ship onJune 05, 2026Details

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biological source

mouse

Quality Segment

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

4G10®, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG2bκ

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr)

General description

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Application

Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.

Physical form

Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10 (FOX-NY [NS-1 derivative] myeloma x spleen cells).

Analysis Note

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Routinely evaluated on EGF-treated human A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
16-10316-20416-104
antibody form

purified from hybridoma cell culture

antibody form

purified antibody

antibody form

purified antibody

antibody form

-

clone

4G10®, monoclonal

clone

4G10®, monoclonal

clone

4G10®, monoclonal

clone

4G10®, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

isotype

IgG2bκ

isotype

IgG2bκ

isotype

IgG2bκ

isotype

-


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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