58934
SUPELCOSIL™ Suplex pKb-100 (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
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product name
SUPELCOSIL™ Suplex pKb-100 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Agency
suitable for USP L68
feature
endcapped: no
manufacturer/tradename
SUPELCOSIL™
extent of labeling
12.5% carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 3.4 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
Suplex pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL LC-ABZ columns. These specially deactivated columns differ in that Suplex pKb-100 is not endcapped, while SUPELCOSIL LC-ABZ is endcapped. The absence of the end-capping reagent results in better performance from Suplex pKb-100 for the strongest basic compounds, while LC-ABZ is preferred when the sample also contains acids and/or zwitterions.
Application
SUPELCOSIL™ Suplex pKb-100 HPLC Column was used in HPLC purification of prepurified fly ovaries. It was also used for HPLC analysis, done for the determination of retinoid.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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