This product may be stored at -20°C in aliquots at least until the recommended retest date published in the Certificate of Analysis. Avoid repeated freeze/thaw cycles.
T1952
Trichostatin A, Ready Made Solution
5 mM in DMSO, from Streptomyces sp.
Synonym(s):
TSA, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
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About This Item
Empirical Formula (Hill Notation):
C17H22N2O3
CAS Number:
Molecular Weight:
302.37
UNSPSC Code:
12352202
NACRES:
NA.32
Pricing and availability is not currently available.
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biological source
Streptomyces sp.
Quality Level
Assay
≥98% (HPLC)
form
DMSO solution
concentration
5 mM in DMSO
technique(s)
cell culture | mammalian: suitable
antibiotic activity spectrum
fungi
neoplastics
Mode of action
enzyme | inhibits
shipped in
dry ice
storage temp.
−20°C
SMILES string
N(O)C(=O)\C=C\C(=C\C(C)C(=O)c1ccc(cc1)N(C)C)\C
InChI
1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
InChI key
RTKIYFITIVXBLE-WKWSCTOISA-N
General description
Trichostatin A is a compound of primary hydroxamic acid.[1]
Application
Trichostatin A, ready-made solution has been used:
- as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing[2]
- to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)[3]
- to treat cells for the HDAC inhibition experiments[4]
Biochem/physiol Actions
Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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