After reconstitution with water. For extended storage, it is recommended to further dilute the solution in a buffer containing a carrier protein (example 0.1% BSA) and store it in working aliquots at -20°C to -80°C. As BSA is a significant component of FBS this would meet the long-term storage recommendation criteria.
SRP3098
IL-8 (77aa) (CXCL8) human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
CXCL8, NAP-1, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF)
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Pricing and availability is not currently available.
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biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
8.9 kDa
packaging
pkg of 25 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IL8(3576)
General description
Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that can signal through the C-X-C motif chemokine receptors 1 and 2 (CXCR1 and 2). It is also called as C-X-C motif chemokine ligand 8 (CXCL8).[1][2] IL-8 is secreted by monocytes and endothelial cells. The gene encoding IL-8 is localized on human chromosome 4q13.3.[3] Recombinant human IL-8 (endothelial-derived) is an 8.9kDa protein containing 77 amino acid residues.
Application
IL-8 (77aa) (CXCL8) human has been used as a G protein-coupled receptor (GPCR) agonist.[4]
Biochem/physiol Actions
Sequence
AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
Physical form
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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AFTER THE INITIAL RECONSTITUTION IN WATER, CAN THIS BE DILUTED IN PBS WITH 10% FBS?
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