The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.
Unit Definition
One unit will bind 1 μg biotin.
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Using optical tweezers and single particle tracking, we have revealed the motion of a single protein, the lambda-receptor, in the outer membrane of living Escherichia coli bacteria. We genetically modified the lambda-receptor placing a biotin on an extracellular site of
Journal of cellular physiology, 235(2), 1141-1154 (2019-07-28)
Ovarian cancer (OC) is a fatal cancer in women, mainly due to its aggressive nature and poor survival rate. The lncRNA-miRNA-mRNA (long noncoding RNA-microRNA-messenger RNA) interaction is promising biomarkers for the improving prognosis of OC. Therefore, we explored the regulatory
The motion of a single molecule, the lambda-receptor, in the bacterial outer membrane.
Oddershede L et al
Biophysical Journal, 83(6), 3152-3161 (2002)
Tetrahydropteridines suppress gene expression and induce apoptosis of activated RAW264.7 cells via formation of hydrogen peroxide.
Biochimica et biophysica acta. General subjects, 1863(10), 1471-1479 (2019-05-24)
Sialylation of glycoproteins and glycolipids is important for biological processes such as cellular communication, cell migration and protein function. Biosynthesis of CMP-sialic acid, the essential substrate, comprises five enzymatic steps, involving ManNAc and sialic acid and their phosphorylated forms as
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