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RAB0098

Sigma-Aldrich

Human M-CSF / CSF1 ELISA Kit

for serum, plasma, cell culture supernatant, urine

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.78-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CSF1(1435)

General description

M-CSF (colony stimulating factor 1) is a pleiotropic macrophage growth factor. It has a signal peptide, a 149-amino acid receptor-binding domain, a variable spacer region and a transmembrane region with a cytoplasmic tail. The human M-CSF receptor is a 972-amino acid glycosylated protein which consist of an extracellular region made of five immunoglobulin-like domains (D1–D5), a transmembrane region and an intracellular autocatalytic kinase domain. This gene is mapped to human chromosome p13-p21.
The Human MCSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MCSF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human MCSF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

M-CSF (colony stimulating factor 1) plays a major role in the immune response, bone metabolism and the development of some cancers. It modulates macrophage survival, proliferation, differentiation and chemotaxis.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Combinatorial and computational approaches to identify interactions of macrophage colony-stimulating factor (M-CSF) and its receptor c-FMS.
Rosenfeld L, et al.
The Journal of Biological Chemistry, 290(43), 26180-26193 (2015)
Teresa Di Desidero et al.
Pharmacological research, 158, 104920-104920 (2020-05-29)
Anaplastic thyroid cancer (ATC) is a rare neoplasia with a poor prognosis. Proliferation and apoptosis assays were performed on ATC cell lines (8305C, 8505C) exposed to vinorelbine, lenvatinib, as well as to concomitant combinations. ABCB1, ABCG2 and CSF-1 mRNA expression
Reassignment of the human CSF1 gene to chromosome 1p13-p21.
Morris SW, et al.
Blood, 78(8), 2013-2020 (1991)

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