P7173
Pyruvate Carboxylase from bovine liver
buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)
Synonym(s):
Pyruvate:CO2 ligase (ADP-forming)
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.26
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form
buffered aqueous glycerol solution
specific activity
5-25 units/mg protein (BCA)
concentration
≥0.5 mg/mL
foreign activity
lactic dehydrogenase ≤0.5%
storage temp.
−20°C
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Application
Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.
Biochem/physiol Actions
Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.
Unit Definition
One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 °C.
Physical form
Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.
Preparation Note
Affinity purified
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Helle Mark Sickmann et al.
Neurochemistry international, 60(3), 267-275 (2012-01-17)
The number of people suffering from diabetes is hastily increasing and the condition is associated with altered brain glucose homeostasis. Brain glycogen is located in astrocytes and being a carbohydrate reservoir it contributes to glucose homeostasis. Furthermore, glycogen has been
Xiaoxia Yin et al.
Applied microbiology and biotechnology, 96(6), 1527-1537 (2012-06-09)
In previous research, a thiamine-auxotrophic yeast for alpha-ketoglutaric acid (KGA) overproduction was screened in our laboratory and named Yarrowia lipolytica WSH-Z06 (CCTCC no. M207143). However, the high concentration of by-products (mainly pyruvate) limited its application on an industrial scale. To
Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum.
Peters-Wendisch, Petra G., et al.
Microbiology, 143(4), 1095-1103 (1997)
Abdussalam Adina-Zada et al.
Biochemistry, 51(41), 8208-8217 (2012-09-19)
Mutation of Arg427 and Arg472 in Rhizobium etli pyruvate carboxylase to serine or lysine greatly increased the activation constant (K(a)) of acetyl CoA, with the increase being greater for the Arg472 mutants. These results indicate that while both these residues
Philip Lee et al.
Hepatology (Baltimore, Md.), 57(2), 515-524 (2012-08-23)
The pathogenesis of type 2 diabetes is characterized by impaired insulin action and increased hepatic glucose production (HGP). Despite the importance of hepatic metabolic aberrations in diabetes development, there is currently no molecular probe that allows measurement of hepatic gluconeogenic
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