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N9784

Sigma-Aldrich

Neutralization Solution for Blood

sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

Synonym(s):

DNA neutralization solution, Neutralization buffer DNA extraction

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

liquid

usage

sufficient for 100 reactions
sufficient for 1000 reactions

storage temp.

room temp

General description

Neutralization Solution for Blood is used along with lysis solution for blood (L3289) for the rapid extraction and neutralization of human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This solution is a component of the Extract-N-Amp and the REDExtract-N-Amp Blood PCR Kits

Application

Neutralization Solution for blood has been used to neutralize extracted DNA prior to polymerase chain reaction (PCR) amplification.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made
Sabrina M Rodriguez et al.
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Amber K Bowers et al.
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To take advantage of available expressed sequence tags and genomic sequence, we have developed 64 PCR-based molecular markers in Chlamydomonas reinhardtii that map to the 17 linkage groups. These markers will allow the rapid association of a candidate gene sequence

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