N8630

Nuclease P₁ from Penicillium citrinum

lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)

• Synonym(s): 3′-Phosphohydrolase, Nuclease 5′-Nucleotidehydrolase, Endonuclease P₁
• CAS Number: 54576-84-0
• Enzyme Commission number: 3.1.30.1 (BRENDA, IUBMB)
• MDL number: MFCD00131010
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Penicillium citrinum
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
• secondary activity: ≥1,000 units/mg protein 3′-nucleotidase
• mol wt: 42-50 kDa
• packaging: vial of ≥250 units (using RNA substrate)
• technique(s): DNA extraction: suitable; DNA purification: suitable
• suitability: suitable for molecular biology
• application(s): cell analysis
• storage temp.: 2-8°C

Description

General description

Nuclease P1 is one of the most commonly known single-strand specific nucleases in molecular biology; Nuclease P1 is a single stranded specific endoduclease (of ssDNA or ssRNA). Nuclease P1 can also cleave single-stranded regions in double-stranded nucleic acids.
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. [1] Nuclease P1 selective activity has found useful applications in studies on nucleic acid structure.

Application

• Nuclease P1 cleave of single stranded DNA or RNA to 5′ mononucleotides
• Nuclease P1 supports DNA damage and modification research
• Nucleic acids base composition and structural analysis can be done by Nuclease P1
• Nuclease P1 has historically been used for the industrial production of 5′-mononucleotides from yeast RNA.
• Removal of nucleic acids through protein purification can be done by Nuclease P1
• Nuclease P1 is a key reagent for the development of methods for studies involving t-RNA dependent amino acid biosynthesis and t-RNA dependent trans-amidation
• Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent.
• Nuclease P1 was used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA.
• Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.

The enzyme has an optimal temperature of approximately 70°C, but for a long incubation, a temperature below 60°C is more suitable. It is stable in the pH range of 5 - 8.

The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.

Biochem/physiol Actions

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.

Features and Benefits

Our highly active Nuclease P1 is tested for its 3′- 5′ - Phosphodiesterase Activity and 3′- Nucleotidase Activity and is the most active Nuclease P1 in the market

Physical properties

A zinc dependent glycoprotein consisting of 270 amino acid residues. Molecular mass: 42-50 kDa.

Unit Definition

3′-5′-Phosphodiesterase: One unit will liberate 1.0 μmole of acid soluble nucleotides from RNA per min at pH 5.3 at 37 °C.
3′-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3′-AMP per min at pH 7.2 at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves