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MBD0043

Sigma-Aldrich

Vitamin K1-Hemin ready made solution

For bacterial culture

Synonym(s):

anaerobic media supplement

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.74

Quality Level

concentration

-100 ×

technique(s)

FISH: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Hemin- Vitamin K1 readymade solution is used as a culture medium enrichment for fastidious anaerobic microorganisms.1,2 Hemin-Vitamin K1 is a key ingredient compound that is part of anaerobic media such as brucella broth, thioglycolate broth, reinforced clostridial medium, cooked meat broth, peptone yeast extract medium, fastidious anaerobe agar etc. Hemin helps to regulate the expression of numerous virulence factors and extracellular vesicle formations in Porphyromonas sp. 3,4. Vitamin K1 (phylloquinone) is produced by plants, it is used as a redox cofactor in plants and cyanobacteria. Vitamin K1 is converted into vitamin K2 in the gut by intestinal microbiota. Hemin and Vitamin K1have been shown to enhance the growth of many anaerobes including Bacteroides species and certain gram-positive non-spore formers organisms.

Application

Hemin- Vitamin K1 readymade solution is suitable to use as a media supplement for anaerobic bacterial culture .

Features and Benefits

  • Enriches fastidious anaerobic microorganisms
  • Ready-made solution
  • Sterile filtered

Other Notes

For each liter (L) of medium prepared, add 10-20 mL Hemin Vitamin K readymade solution after autoclaving.

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

120.2 °F

Flash Point(C)

49 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J M Conly et al.
Progress in food & nutrition science, 16(4), 307-343 (1992-10-01)
Vitamin K is an essential cofactor necessary for the production of clotting factors II, VII, IX, and X in humans and has recently been found to be an essential factor for many other proteins in the body. There are two
Teresa Olczak et al.
FEMS microbiology reviews, 29(1), 119-144 (2005-01-18)
Porphyromonas gingivalis is a Gram-negative anaerobic bacterium associated with the initiation and progression of adult periodontal disease. Iron is utilized by this pathogen in the form of heme and has been shown to play an essential role in its growth
Best Practices for the Pre-Analytic Phase of Anaerobic Bacteriology
Clinical microbiology newsletter, 44(7), 63-71 (2022)
Vitamin K and Hallmarks of Ageing: Focus on Diet and Gut Microbiome
Nutrients, 12, 2727-2727 (2023)
P D Marsh et al.
Microbiology (Reading, England), 140 ( Pt 4), 861-865 (1994-04-01)
Porphyromonas gingivalis strain W50 was grown under haemin-limitation and haemin-excess conditions in a chemostat at pH 7.5. The maximum specific growth rate (mumax) was determined at both haemin concentrations (mumax = 0.236 +/- 0.052 and 0.271 +/- 0.039 h-1, respectively).

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