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K1757

Sigma-Aldrich

Keratinocyte Growth Factor human

KGF, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):

hFGF-7, hKGF, FGF-7, KGF

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About This Item

CAS Number:
148348-15-6
MDL number:
MFCD00212245
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE and HPLC)

form

powder

potency

0.1-15 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

18.9 kDa

packaging

pkg of 10 and 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

1 EU/μg

UniProt accession no.

P21781

storage temp.

−20°C

Gene Information

human ... FGF7(2252)

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General description

Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids, belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines, mesenchymal cells but absent from normal glial cells and epithelial cell lines.

Application

Keratinocyte Growth Factor human has been used:
  • as a component of epithelial growth medium
  • as a positive control for antigen to determine the activity of the antibody
  • to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions

Biochem/physiol Actions

Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium. It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells. KGF contributes to the survival of cells under stress conditions. KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

Determined by a cell proliferation assay using 4MBr-5 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stimulation of in vivo angiogenesis by in situ crosslinked, dual growth factor-loaded, glycosaminoglycan hydrogels
Elia R, et al.
Biomaterials, 31(17), 4630-4638 (2010)
Synergism between keratinocyte growth factor and carboxymethyl chitosan reduces pericardial adhesions
Lopes JB, et al.
The Annals of Thoracic Surgery, 90(2), 566-572 (2010)
Characteristics of MIC-1 antlerogenic stem cells and their effect on hair growth in rabbits.
Cegielski M, et al.
In Vivo, 27(1), 97-106 (2013)
Nrf2 transcription factor, a novel target of keratinocyte growth factor action which regulates gene expression and inflammation in the healing skin wound
Braun S, et al.
Molecular and Cellular Biology, 22(15), 5492-5505 (2002)
Keratinocyte growth factor stimulates migration and hyaluronan synthesis in the epidermis by activation of keratinocyte hyaluronan synthases 2 and 3
Karvinen S, et al.
The Journal of biological chemistry, 278(49), 49495-49504 (2003)
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