The choice of loading dye will not impact the performance the the E3004 - Extract-N-Amp™ PCR ReadyMix. While we have not tested loading dyes outside of the product, G2526, any commercial DNA loading dye is expected to be compatible.
E3004
Extract-N-Amp™ PCR ReadyMix™
Extract-N-Amp™ PCR ReadyMix™
Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
Synonym(s):
Plant direct PCR master mix, Tissue direct PCR master mix
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About This Item
UNSPSC Code:
41106303
NACRES:
NA.55
Pricing and availability is not currently available.
Recommended Products
Quality Level
usage
sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications
feature
dNTPs included
hotstart
color
colorless
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
General description
Extract-N-Amp™ PCR ReadyMix™ is a specially formulated hot start PCR master mix for direct PCR amplification from the extract.
PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed.
Application
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
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Can I use the TriTrack DNA Loading Dye or the TrakitCyanoOrange Loading Buffer with the ReadyMix??
1 answer-
Helpful?
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What is the polymerase used for this kit? I am trying to calculate Tm for my PCR, but I would need to know what type of polymerase this kit uses. I am guessing something close to Q5 Hotstart?
1 answer-
This kit employs Taq DNA polymerase (Product D6677).
Helpful?
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