Anti-DNP antibody produced in rabbit was used to tag derivatized protein carbonyl groups in plasma by ELISA. It was used for differential cell count of zona-free blastocysts in IVF embryos.
Biochem/physiol Actions
2,4-Dinitrophenyl is an oxidative phosphorylation inhibitor and hinders adenosine triphosphate (ATP) synthesis. Due to its low excretion rate, 2,4-dinitrophenyl is considered toxic to human. It increases electron transportation, respiration and overall metabolism.
Analysis Note
Antiserum is tested for specificity and titer by immunoelectrophoresis; treated to remove lipoproteins..
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Aging increases the risk of type 2 diabetes, and this can be prevented by dietary restriction (DR). We have previously shown that DR inhibits the downregulation of miRNAs and their processing enzymes - mainly Dicer - that occurs with aging
In organisms with a soma-germ demarcation, the germline must be 'preserved' such that harmful damage is not transmitted to the offspring. Keeping the progeny free of damage may be achieved by gametes enjoying elevated, and/or more functional, homeostatic maintenance systems.
The Well of the Well (WOW) system has been developed to culture embryos in small groups or to track the development of single embryos. In the present study, we aimed to examine the effects of the microenvironment provided by the
Journal of the American Heart Association, 5(10) (2016-10-04)
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