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RAW 264.7 Cell Line murine
91062702, mouse blood, Macrophage
Synonym(s):
RAW264 7 Cells, RAW264.7 Cells
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UNSPSC Code:
41106514
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Product Name
RAW 264.7 Cell Line murine, Macrophage from blood, 91062702
biological source
mouse blood
growth mode
Semi-adherent
karyotype
Not specified
morphology
Macrophage
products
Lysozyme
receptors
Immunoglobulin, complement
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse monocyte macrophage
Cell Line Description
Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.
Application
Metabolic studies
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.
Other Notes
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Are my Cell Line cells mycoplasma tested?
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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How do I handle Cell Line frozen cells upon arrival?
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Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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